Transformation frequency by simian papovavirus 40 of fibroblasts from a lung cancer patient with Klinefelter's syndrome and XY/XXY mosaicism was 3 to 10 times higher than that of fibroblasts from individuals with normal
نویسندگان
چکیده
Transformation frequency by simian papovavirus 40 of fibroblasts from a lung cancer patient with Klinefelter's syndrome and XY/XXY mosaicism was 3 to 10 times higher than that of fibroblasts from individuals with normal karyotypes and no history of cancer. The patient's XXY cell strain also manifested a 3-fold increase in susceptibility to transformation as compared to his XY cell strain. This observation, coupled with the finding of a high frequency of sex chromatin-positive cells in his tumor, could indicate origin of the tumor from the aneuploid cell population.
منابع مشابه
Increased Susceptibility of Cells from Cancer Patients with XV-Gonadal Dysgenesis to Simian Papovavirus 40 Transformation
Genetically susceptible cells in culture can be transformed by oncogenic viruses. Quantitative assay methods have been developed for estimating the frequency of transformation. In an attempt to estimate the susceptibility to transformation of cells from cancer patients with XY-gonadal dysgenesis, fibroblasts from two such patients were infected with simian papovavirus 40, and transformation fre...
متن کاملIncreased susceptibility of cells from cancer patients with XY-gonadal dysgenesis to simian papovavirus 40 transformation.
Genetically susceptible cells in culture can be transformed by oncogenic viruses. Quantitative assay methods have been developed for estimating the frequency of transformation. In an attempt to estimate the susceptibility to transformation of cells from cancer patients with XY-gonadal dysgenesis, fibroblasts from two such patients were infected with simian papovavirus 40, and transformation fre...
متن کاملEvaluation of silica nanoparticles cytotoxicity (20-40 nm) on cancerous epithelial cell (A549) and fibroblasts cells of human normal lung fibroblast (MRC5)
Introduction: Silica nanoparticles have received more attraction in medical and industrial applications due to their unique properties such as small size, the possibility of surface functionalization, ease of production, and low cost. So, it is necessary to study the respiratory toxicity of occupational exposure due to the production and increasing use of silica nanoparticles, especially in the...
متن کاملAssessment of sex chromosome aneuploidy in sperm nuclei from 47,XXY and 46,XY/47,XXY males: comparison with fertile and infertile males with normal karyotype.
Sex chromosome aneuploidy was assessed in spermatozoa from a 47,XXY male and a 46,XY/47,XXY male using three colour fluorescence in-situ hybridization (FISH) and compared with two control groups. The first group included subjects of proven fertility and the second infertile males with normal constitutional karyotype. The frequencies of XX and YY disomic, XY hyperhaploid and diploid spermatozoa ...
متن کاملReport of a Case with Trisomy 9 Mosaicism
Trisomy 9 is a rare chromosome disorder with high neonatal mortality. It is often seen in mosaic form. Most patients who survive are severely mentally retarded. The main features of this syndrome are "bulbous" nose, microphthalmia, dislocated limbs, and other anomalies of skeletal, cardiac, genitourinary, and central nervous system. Most patients have developmental and cognitive impairment. Pat...
متن کامل